Open AccessIsolation of Sulfated Anticoagulant Compound from Fermented Red Seaweed Grateloupia filicina
Author(s) -
Nikapitiya Chamilani,
De Zoysa Mahanama,
Jeon YouJin,
Lee Jehee,
Jee Youngheun
Publication year - 2007
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2007.00112.x
Subject(s) - chromatography , sulfation , biology , polysaccharide , fermentation , biochemistry , chemistry
The red alga Grateloupia filicina was fermented with 0.15 g/mL sucrose at 25 C, and anticoagulant activity was measured biweekly up to the 10th wk by activated partial thromboplastin time (APTT). A sulfated polysaccharide compound having anticoagulant activity was purified from the sixth wk of fermentation by fast protein liquid chromatography using diethylaminoethyl cellulose followed by a sepharose‐4B column. The concentration of the purified sulfated anticoagulant polysaccharide was 136.65 μg/mL, and the sulfate concentration of the purified compound was 26.33 μg/mL. The presence of just a single spot on agarose gel electrophoresis confirmed that the compound was purified, and polyacrylamide gel electrophoresis showed that the purified compound was of high molecular mass. The purified sulfated anticoagulant polysaccharide consisted of galactose, glucose, and mannose in an approximate molar ratio of 0.95 : 0.01 : 0.03. Both purified anticoagulant and commercial heparin showed >1000 sec APTT activity at 136.65 μg/mL. As the technique of fermentation is inexpensive, the purified anticoagulant compound could have significant potential in the medical and pharmaceutical industry.