Open AccessKinetics of Cellular Component in Inflammatory Response Induced by Different Stimuli in the Swim Bladder of Pacu, Piaractus mesopotamicus Holmberg 1887 (Characidae)
Author(s) -
Bozzo F. R.,
Moraes J. R. E.,
Ruasde Moraes Flávio,
Pereira G. T.,
TavaresDias M.,
Onaka E. M.
Publication year - 2007
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2007.00100.x
Subject(s) - piaractus mesopotamicus , biology , characidae , lipopolysaccharide , pacu , inoculation , aeromonas hydrophila , inflammation , microbiology and biotechnology , midgut , andrology , zoology , immunology , botany , fish <actinopterygii> , fishery , medicine , larva
.— The present study assessed the kinetics of cell accumulation at the site of inflammation induced by thioglycolate, Escherichia coli lipopolysaccharide (LPS) and heat‐inactivated Aeromonas hydrophila , in the pacu, Piaractus mesopotamicus (Characidae), swim bladder. A quantitative, as well as qualitative, assessment was done of all the cells present in the exudate at 6, 24, and 48 h ( n = 8) after inoculation of inflammatory agents. The results show that the thioglycolate was the irritant to induce higher total inflammatory cell accumulation when compared to the control group, 6 h after insult ( P < 0.05). Inoculation of heat‐inactivated Aeromonas hydrophila induced progressive accumulation of total inflammatory cells, with cell number peaking after 24 h and being significantly higher than observed in the other groups ( P < 0.05). Injection of LPS also induced greater cell accumulation when compared to the control group ( P < 0.05), although in lower numbers than those induced by the other two irritants. All irritants injected induced significantly greater accumulation of lymphocytes and thrombocytes when compared to the control group ( P < 0.05).