Open AccessAnalysis of Digestive Gland Expressed Sequence Tag Library from the Disk Abalone, Haliotis discus discus
Author(s) -
Munasinghe Helani,
Kang Hyun Sil,
Lee Jehee
Publication year - 2006
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2006.00012.x
Subject(s) - biology , abalone , expressed sequence tag , haliotis discus , gene , cdna library , annotation , genetics , dna sequencing , identification (biology) , sequence (biology) , computational biology , complementary dna , fishery , botany
As a first step toward the functional annotation of unknown genes in the disk abalone, Haliotis discus discus , 900 clones from an abalone digestive gland complementary DNA library were selected for partial sequencing from the 5′ end. A total of 841 clones were successfully sequenced and assembled into 122 clusters and 510 singletons using TIGR Assembler. Each of the identified clusters consisted of at least two expressed sequence tags (ESTs), with each cluster considered to be derived from the same gene. The 122 clusters contained a total of 331 sequences. The 632 unique transcripts were compared against the National Center for Biotechnology Information databases by BlastX and BlastN programs. Out of these unique transcripts, 354 were significantly ( E < 10 −5 ) similar to sequences present in the databases. Therefore, this study identified and sequenced 278 putative novel transcripts. The ESTs generated in this study will be useful both as molecular markers and as a resource for the identification of novel genes.