Open AccessComparison of the Coat Protein Gene of Nervous Necrosis Virus (NNV) Detected from Marine Fishes in Korea
Author(s) -
Oh MyungJoo,
Jung SungJu,
Kitamura ShinIchi
Publication year - 2005
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2005.tb00389.x
Subject(s) - biology , olive flounder , grouper , virus , polymerase chain reaction , flounder , gene , coat protein , virology , paralichthys , anatomy , microbiology and biotechnology , fishery , zoology , fish <actinopterygii> , genetics , rna
Marine fishes, red drum Sciunops ocellatus . oblong rock fish Sebastes oblongus and Japanese flounder Purulichthys olivaceus showing clinical signs of nodavirus infection and mortality were subjected to histopathological and reverse‐transcription polymerase chain reaction (RT‐PCR) detection assay. Histologically, the diseased fish showed prominent vacuolisation in the brain, spinal cord, and retina of eyes. Fish nodavirus was detected by RT‐PCR amplification in these fish species. Sequence analysis of the RT‐PCR products were identical in comparison among T4 regions of Japanese flounder, red drum, and oblong rock fish isolates. From the sequence analysis, three isolates in this study could be included in the red‐spotted grouper nervous necrosis virus (RGNNV), which is one of the clusters among four major clusters of NNV.