Open AccessEffect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense
Author(s) -
Wang WeiNa,
Wang AnLi,
Wang Yue,
Wang Junxia,
Sun RuYong
Publication year - 2005
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2005.tb00124.x
Subject(s) - catalase , superoxide dismutase , glutathione reductase , biology , glutathione peroxidase , vitamin , glutathione , antioxidant , reactive oxygen species , vitamin c , biochemistry , vitamin e , medicine , endocrinology , enzyme
This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia‐N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia‐N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia‐N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia‐N concentration. The glutathione transference activity increased at a low concentration of ammonia‐N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia‐N. This response involved several biochemical reactions known to be involved in cellular defense.