Open AccessCryogenic and Refrigerated Storage of Rainbow Smelt Osmerus mordax Spermatozoa
Author(s) -
DeGraaf J. Dana,
Berlinsky David L.
Publication year - 2004
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2004.tb01076.x
Subject(s) - extender , cryoprotectant , biology , sperm , milt , andrology , human fertilization , sperm motility , smelt , cryopreservation , motility , anatomy , chemistry , botany , embryo , fishery , fish <actinopterygii> , microbiology and biotechnology , medicine , organic chemistry , polyurethane
The effects of extender composition, cryoprotectant, and freezing rate on post‐thaw rainbow smelt Osmerus mordax sperm motility were examined, and the fertilization capacity of fresh and post‐thaw sperm were compared. The highest post‐thaw motility (75%) was obtained when milt was diluted 1:3 with an extender containing 600 mM sucrose supplemented with 10% dimethyl sulfoxide and 1.5% bovine serum albumin and frozen at a rate of –20 C/min. Post‐thaw motility for sperm stored in this extender was similar to fresh sperm and did not change after 90 d of storage. Furthermore, there were no differences in fertilization rate or embryo survival to the eyed stage between fresh and post‐thaw sperm frozen in this extender. The lowest post‐thaw motility was observed when sperm were frozen with methanol at a rate of ‐30 C/min. Refrigerated sperm diluted 1:3 with the 600 mM sucrose extender remained motile for 30 d. These data demonstrate that rainbow smelt spermatozoa can be effectively used following short and long‐term storage using a simple, sucrose‐based extender.