Open AccessGenetic Effects for Response to Live Edwardsiella ictaluri, Killed E. ictaluri, and Stress in Juveniles from All Crosses Among USDA 103, USDA 102, and Norris Channel Catfish Ictalurus punctatus Strains
Author(s) -
Bosworth Brian G.,
Wolters William R.,
Wise David J.,
KLESIUS P. H.
Publication year - 2004
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.2004.tb01062.x
Subject(s) - ictalurus , edwardsiella ictaluri , catfish , biology , purebred , zoology , veterinary medicine , antibody , crossbreed , fish <actinopterygii> , fishery , immunology , medicine
Juveniles from all possible crosses among USDA 102. USDA 103, and Norris channel catfish Ictalurus punctatus strains were compared for: 1) survival and mix‐Edwardsiella ictaluri antibody after exposure to live E. ictaluri bacterium (isolate 597‐458); 2) antibody level after injection with formalin‐killed E. ictaluri (597‐458); and 3) pre‐stress. post‐stress, and stress‐recovery serum Cortisol levels. Purebred and crossbred USDA 102 strain fish had higher survival (mean of five genetic groups = 87%) and lower anti‐ E.ictaluri antibody (mean optical density (OD) of five genetic groups = 0.167) 30 d after live E. ictaluri challenge than purebred Norris and USDA 103 strains and their crosses (means of four genetic groups = 60% survival and 0.210 OD antibody level). Significant general combining ability, line effects, and heterosis indicated that the USDA 102 strain contributed additive and dominance genetic effects for increased survival and lower antibody level after live E. ictaluri challenge. Antibody response to formalin‐killed, intra‐peritoneally injected E. ictaluri was not different among genetic groups (overall mean = 0.198 OD). Serum Cortisol was measured prior to (pre‐stress), immediately after (post‐stress), and 2 h after (stress‐recovery) a standard stressor. Serum Cortisol level was highest in post‐stress fish (35.8 ng/mL), intermediate in stress‐recovery fish (10.9 ng/mL), and lowest in pre‐stress fish (6.5 ng/mL), but was not different among genetic groups within a stress time period. Results indicate diat differences exist among genetic groups of channel catfish for survival and antibody production after live E. ictaluri challenge, but these differences were not related to antibody response to killed E. ictaluri or serum Cortisol levels.