Open AccessMulti‐Locus DNA Fingerprinting of Channel Catfish Ictalurus punctatus
Author(s) -
Bosworth Brian G.,
Wolters William R.
Publication year - 1997
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.1997.tb00643.x
Subject(s) - ictalurus , catfish , biology , dna profiling , ictaluridae , locus (genetics) , restriction enzyme , strain (injury) , fish <actinopterygii> , genetics , dna , fishery , gene , anatomy
.— Similarities among multi‐locus DNA fingerprints of five channel catfish Ictalurus punctatus strains and the ability to identify the strain of a fish based on its fingerprint pattern were investigated. Five restriction enzymes and 13 multi‐locus DNA probes were screened to identify enzyme‐probe combination useful for DNA fingerprinting channel catfish. Restriction enzymes Hinf I and Dpn II, in combination with probes (CAC)n, (CGC)n, (CTC)n, (ATCC)n, and (GATA)n, produced useful fingerprints (20–30 resolvable bands for each enzyme‐probe combination). Thirty individuals (3 pools of 10 individuals each) from each of five channel catfish strains (albino, Mississippi normal, USDA‐102, USDA‐102 select, and USDA‐103) were fingerprinted with all useful enzyme‐probe combinations. Band sharing among samples was higher within strains than among strains and band sharing among strains was higher for strains whose breeding history indicated a high degree of relatedness. Individual fingerprints of 18 fish from each of the USDA‐102 select and USDA‐103 strains revealed no strain‐specific bands, but several diagnostic bands (present at high frequencies in either USDA‐102 select or USDA‐103 strains and at a low frequencies in other strains) were identified. Band sharing at diagnostic bands was used to correctly identify fish as USDA‐102 select or USDA‐103 strains with 82% accuracy from fingerprints of 17 USDA‐102 select strain fish, 18 USDA‐103 strain fish, and 38 fish collected from three commercial farms.