Open AccessInduced Ovulation of Southern Flounder Paralichthys lethostigma Using Gonadotropin Releasing Hormone Analogue Implants
Author(s) -
Berlinsky David L.,
King V William,
Smith Ted I. J.,
Hamilton Richard D.,
Holloway John,
Sullivan Craig V.
Publication year - 1996
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.1996.tb00263.x
Subject(s) - ovulation , biology , flounder , follicle , andrology , hormone , gonadotropin releasing hormone , medicine , endocrinology , zoology , fish <actinopterygii> , fishery , luteinizing hormone
Implanted pellets that provide a sustained release of [D‐Ala 6 Des‐Gly 10 ] LHRH‐ethylamide (GnRHa) were used to induce maturation and ovulation of Southern flounder Paralichthys lethostigma . Of the 12 females whose ovaries contained follicles with a maximum diameter ≥500 μm, 11 ovulated for the first time within 90 h of hormone implantation. Only 1 fish with a maximum follicle diameter less than 500 μm ovulated within 2 wk after implantation. Ovulated eggs were manually stripped from the females and mixed with sperm from several males. Most females were spawned 1 to 3 times on consecutive days with variable fertility. One female was spawned 11 times producing 668,000 eggs. Fertility was evaluated by examining the incubated eggs for early stages of embryonic cleavage. The percentage of fertile eggs in subsamples of incubated eggs ranged from 7–95%. The results indicate that GnRHa implants can be used to induce repeated ovulation in this species. The variability in fertility is discussed in relation to egg quality.