Open AccessManipulations of Shrimp Embryos: A Viable Technique for the Removal of the Hatching Envelope
Author(s) -
Lynn John W.,
Glas Patricia S.,
Hertzler Philip L.,
Clark Wallis H.,
Green Jeffrdy D.
Publication year - 1993
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.1993.tb00143.x
Subject(s) - hatching , biology , embryo , envelope (radar) , shrimp , fishery , zoology , andrology , ecology , engineering , telecommunications , radar , medicine
The hatching envelope elevated around the spawned eggs of penaeoidean eggs presents a formidable barrier for manipulation of the embryo. By spawning eggs into seawater containing 3‐amino‐1,2,4‐triazole and subsequently passing the eggs through Nitex screening approximately 45 min after spawning, the hatching envelope can be effectively removed with little or no damage to the embryos. The time and extent to which the envelope is removed can be varied. Embryos treated in this manner continue to develop and the method provides a new technique with which investigators can gain access to the surface of the egg or embryo.