Open AccessThe Induction of Tetraploidy in Salmonids
Author(s) -
Myers James M.,
Hershberger William K.,
Iwamoto Robert N.
Publication year - 1986
Publication title -
journal of the world aquaculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0893-8849
DOI - 10.1111/j.1749-7345.1986.tb00546.x
Subject(s) - salmo , biology , rainbow trout , oncorhynchus , chinook wind , zoology , fishery , embryo , cleavage (geology) , andrology , fish <actinopterygii> , medicine , paleontology , fracture (geology)
The induction of tetraploidy was attempted in three intraspecies crosses (autotetraploid) of sal‐monid: chinook salmon ( Oncorhynchus tshawytscha ), coho salmon ( O. kisutch ) and rainbow trout ( Salmo gairdneri ), and in the interspecies hybrid (allotetraploid) coho × Atlantic salmon ( Salmo salar ). Pressure treatments of 6.2 × 10 4 kPa (9,000 psi) were applied for six minutes at intervals from 50% of the time to cleavage to beyond first cleavage. Analysis of eyed stage embryos via flow cytometry indicated two periods of tetraploid induction existed corresponding to 55–75% and 100–110% of the first cleavage interval (FCI). The effects of different pressures and the times of application were analyzed in a study with rainbow trout. Treatments of 4.8 × 10 4 kPa (7,000 psi) for eight minutes, and 5.5 and 6.2 × 10 4 kPa (8,000 and 9,000 psi) for four minutes, were applied at 65, 78, and 100% FCI. Tetraploids were found in the 5.5 and 6.2 × 10 4 kPa group treatments only when applied at 65% FCI. The results indicate that, while tetraploidy is inducible in salmonids, further experimentation is necessary to discern the appropriate time and treatment for optimal tetraploid induction in each species.