Open AccessA QUANTITATIVE ANALYSIS OF DIGESTIVE ENZYMES FOR THE FRESHWATER PRAWN Macrobrachium rosenbergii
Author(s) -
Lee Phillip G.,
Blake Norman J.,
Rodrick Gary E.
Publication year - 1980
Publication title -
proceedings of the world mariculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0748-3260
DOI - 10.1111/j.1749-7345.1980.tb00133.x
Subject(s) - macrobrachium rosenbergii , prawn , hepatopancreas , amylase , biology , food science , biochemistry , trypsin , pepsin , digestive enzyme , digestion (alchemy) , enzyme , shellfish , esterase , fishery , chemistry , aquatic animal , fish <actinopterygii> , chromatography
The specific activities of 10 different digestive enzymes, taken from the hepatopancreas of the freshwater prawn ( Macrobrachium rosenbergii ), were determined. The prawns were 70–110 mm in length and were fed Purina Marine Ration #25 daily. The extracts of M. rosenbergii hepatopancreas exhibited significant activity for the following enzymes: amylase, trypsin, chymotrypsin, pepsin, carboxypeptidase A and B, and leucine aminopeptidase. In addition, the lipolytic enzymes exhibited high esterase activity and only a trace of lipase activity. From this enzyme profile M. rosenbergii is appropriately classified an omnivore. The levels of specific activity reveal the prawn's ability to effectively hydrolyze Purina Marine Ration #25 and further implies that increased protein and starch levels would still be hydrolyzed efficiently. Although individual differences in activities were demonstrated, no direct relationship to sex or size could be detected. In summary, studies concerning the digestive physiology of M. rosenbergii may provide a more logical and efficient approach to its dietary requirements for mariculture.