Open AccessENHANCEMENT OF CHILL TOLERANCE IN LARVAI Artemia salina : COOLING REGIMENS AND MULTIPLE TYPE CRYOPROTECTANT EXPOSURE
Author(s) -
Baust J. G.,
Lawrence A. L.
Publication year - 1979
Publication title -
proceedings of the world mariculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0748-3260
DOI - 10.1111/j.1749-7345.1979.tb00037.x
Subject(s) - cryoprotectant , glycerol , sucrose , larva , biology , zoology , chemistry , cryopreservation , food science , botany , biochemistry , fishery , embryo
The enhancements of chill tolerance of Artemia salina to single (glycerol, glucose or sucrose) and double (glucose‐sucrose) component cryoprotectant solutions were determined. Zero, three, six, twelve and twenty‐four hour post‐hatch larvae were immersed in ‐1°C cryoprotectant solutions for periods up to 90 minutes. The ability of different larval stages to survive an instantaneous change from 28° to ‐1° versus a slow cooling regiment (1°C/hr) from 28° to 18° followed by instantaneous cooling to ‐1°C were compared. Most cryoprotectant solutions showed definite enhancement of survival with the multiple better than single component systems. Survival exceeded 200–500% over ‐1°C cryoprotectant free controls. The significance of these results to the development of cryogenic larval banking techniques is discussed.