Open AccessCONVENTIONAL AND EXPERIMENTAL APPROACHES TO HYBRIDIZATION AND INBREEDING RESEARCH IN THE OYSTER
Author(s) -
Stiles S.
Publication year - 1978
Publication title -
proceedings of the annual meeting ‐ world mariculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 0164-0399
DOI - 10.1111/j.1749-7345.1978.tb00275.x
Subject(s) - crassostrea , biology , hybrid , oyster , inbreeding , human fertilization , heterosis , sperm , larva , interspecific hybridization , zoology , interspecific competition , botany , ecology , genetics , population , demography , sociology
Experimental manipulation of oyster eggs and cleavages is being used to support conventional research on inbreeding and hybridization. Twelve crosses of foundation stock in the commercial American oyster ( Crassostrea virginica ) were made to establish traditional full‐sib lines. In an experimental approach to producing inbreds some parthenogenesis was observed in eggs stimulated with spawned oyster sperm that had been X‐irradiated at doses ranging from 10,000 R to 225,000 R. Geographic and interspecific hybrid crosses with C. virginica have been made. The performance of hybrid larvae stressed with abnormally high and low temperatures was tested. Generally, there was better survival and growth of C. virginica geographic hybrids than of interspecific hybrids with C. gigas . Overall, however, there was better survival and growth of local controls in both experimental hybrid crosses and those crosses made specifically for testing the response of larvae to stress. Cytogenetic data suggested normal fertilization and meiosis in these hybrid crosses. The feasibility of controlling mass fertilization by temporarily inhibiting fertilization in spawning groups intended for hybridization was investigated using EDTA.