Open AccessAN IMPROVED METHOD FOR DETECTING OVARIAN MATURATION IN PINK SHRIMP
Author(s) -
Jannke Thomas E.
Publication year - 1973
Publication title -
proceedings of the annual workshop ‐ world mariculture society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 60
eISSN - 1749-7345
pISSN - 1043-5166
DOI - 10.1111/j.1749-7345.1973.tb00106.x
Subject(s) - shrimp , tris , polyacrylamide gel electrophoresis , chromatography , hemolymph , stain , gel electrophoresis , penaeus , glycine , buffer (optical fiber) , chemistry , ovarian tissue , biology , andrology , microbiology and biotechnology , biochemistry , staining , amino acid , ovary , fishery , enzyme , computer science , medicine , endocrinology , telecommunications , genetics
Polyacrylamide gel electrophoresis was used to separate proteins found in the circulatory fluid of pink shrimp ( Penaeus duorarum ). Because certain proteins in the hemolymph increase in concentration during egg development, the technique can be used to assess the effects of various experimental conditions on ovarian development of experimental shrimp. This method is faster and more sensitive than histological staging of the ova. The best protein separations were produced using a 3 ‐ 5 μ1 sample, 23 C gel‐setting and operating temperature, a 5% gel, a 100V field until the proteins had entered the gel, followed by a 300V field until termination, and Amido Black 10B stain. A 0.43 M Glycine‐Tris buffer (pH = 8.47) and a 0.218 M Tris‐EDTA‐Borate buffer (pH = 8.37) were found to give equal resolution.