Premium
Hematopoietic stem cell expansion: challenges and opportunities
Author(s) -
Walasek Marta A.,
van Os Ronald,
de Haan Gerald
Publication year - 2012
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2012.06549.x
Subject(s) - stem cell , haematopoiesis , progenitor cell , biology , microbiology and biotechnology , ex vivo , transplantation , hematopoietic stem cell , immunology , population , in vivo , medicine , environmental health
Attempts to improve hematopoietic reconstitution and engraftment potential of ex vivo –expanded hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful due to the inability to generate sufficient stem cell numbers and to excessive differentiation of the starting cell population. Although hematopoietic stem cells (HSCs) will rapidly expand after in vivo transplantation, experience from in vitro studies indicates that control of HSPC self‐renewal and differentiation in culture remains difficult. Protocols that are based on hematopoietic cytokines have failed to support reliable amplification of immature stem cells in culture, suggesting that additional factors are required. In recent years, several novel factors, including developmental factors and chemical compounds, have been reported to affect HSC self‐renewal and improve ex vivo stem cell expansion protocols. Here, we highlight early expansion attempts and review recent development in the extrinsic control of HSPC fate in vitro.