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Dental pulp stem cells: osteogenic differentiation and gene expression
Author(s) -
Mori Giorgio,
Brunetti Giacomina,
Oranger Angela,
Carbone Claudia,
Ballini Andrea,
Muzio Lorenzo Lo,
Colucci Silvia,
Mori Claudio,
Grassi Felice Roberto,
Grano Maria
Publication year - 2011
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2011.06234.x
Subject(s) - dental pulp stem cells , junb , osteopontin , stem cell , mesenchymal stem cell , microbiology and biotechnology , osteoblast , cellular differentiation , osteocalcin , biology , alkaline phosphatase , gene expression , chemistry , immunology , gene , in vitro , genetics , biochemistry , enzyme
Dental pulp stem cells (DPSCs) are an adult stem cell population with high proliferative potential and the ability to differentiate in many cell types, and this has led scientists to consider these cells to be an alternative source of postnatal stem cells comparable to mesenchymal stem cells from bone marrow. In this work, we studied the osteoblastic phenotype developed by DPSCs cultured in osteogenic medium. In particular, we analyzed the expression of the typical osteoblast markers such as alkaline phosphatase, collagen type I, osteocalcin, osteopontin, as well as mineralized matrix production. Furthermore, the gene expression during DPSC differentiation into osteoblastic cells was studied by microarray technology. Using microarray and reverse transcriptase–polymerase chain reaction (RT‐PCR) analysis, we found that IGFBP‐5, JunB, and NURR1 genes are upregulated during the differentiation of DPSCs. These data indicate that opportunely differentiated DPSCs show a correct osteoblastic phenotype. Therefore, during the osteoblastic differentiation process, IGFBP‐5, JunB, and NURR1 gene expression is significantly increased.