Premium
Inflammation precedes the development of human malignant mesotheliomas in a SCID mouse xenograft model
Author(s) -
Hillegass Jedd M.,
Shukla Arti,
Lathrop Sherrill A.,
MacPherson Maximilian B.,
Beuschel Stacie L.,
Butnor Kelly J.,
Testa Joseph R.,
Pass Harvey I.,
Carbone Michele,
Steele Chad,
Mossman Brooke T.
Publication year - 2010
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2010.05554.x
Subject(s) - inflammation , neutrophilia , mesothelioma , pathology , cytokine , immune system , peritoneal mesothelioma , medicine , cancer research , immunology
Asbestos fibers cause chronic inflammation that may be critical to the development of malignant mesothelioma (MM). Two human MM cell lines (Hmeso, PPM Mill) were used in a SCID mouse xenograft model to assess time‐dependent patterns of inflammation and tumor formation. After intraperitoneal (IP) injection of MM cells, mice were euthanized at 7, 14, and 30 days, and peritoneal lavage fluid (PLF) was examined for immune cell profiles and human and mouse cytokines. Increases in human MM‐derived IL‐6, IL‐8, bFGF, and VEGF were observed in mice at 7 days postinjection of either MM line, and a striking neutrophilia was observed at all time points. Free‐floating tumor spheroids developed in mice at 14 days, and both spheroids and adherent MM tumor masses occurred in all mice at 30 days. Results suggest that inflammation and cytokine production precede and may be critical to the development of MMs.