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A screen for genes involved in respiration control and longevity in Schizosaccharomyces pombe
Author(s) -
Roux Antoine E.,
Arseneault Geneviève,
Chartrand Pascal,
Ferbeyre Gerardo,
Rokeach Luis A.
Publication year - 2010
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2010.05198.x
Subject(s) - schizosaccharomyces pombe , mutant , gene , yeast , biology , schizosaccharomyces , protein subunit , genetics , phenotype , saccharomyces cerevisiae , life span , microbiology and biotechnology , evolutionary biology
We present results showing that glucose signaling has proaging effects in the yeast Schizosaccharomyces pomb e. Deletion of the receptor that senses extracellular glucose (Git3) increases the life span of S. pombe , while constitutive activation of the Gα subunit acting downstream of this receptor (Gpa2) shortens its life span. The latter mutant is also impaired for growth under respiration conditions. We have used this phenotype in a selection strategy to identify genes that when overexpressed can rescue the respiratory defect of constitutively active Gα subunit mutants. Here, we report an extended version of the work we presented at the IABG meeting and the results of this screen. This strategy allowed us to isolate four genes: psp1 + / moc1 + , cka1 + , adh1 + , and rpb10 + . Interestingly, the overexpression of these genes was also capable of increasing the chronological life span of wild‐type yeast cells.