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Mass spectrometric–based protein chips for detection of food‐derived bioactive components
Author(s) -
Stagsted Jan,
Jørgensen Ann Louise W.,
JuulMadsen Helle R.
Publication year - 2010
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.05258.x
Subject(s) - chemistry , colostrum , computational biology , combinatorial chemistry , nanotechnology , biology , materials science , antibody , immunology
Bioactive components in the diet influence our health and well‐being beyond that of simple supply of energy and raw materials for biochemical reactions. However, the complex chemistry and composition of our food does not make the identification of potential bioactive components a straightforward task. Bioassays for a multitude of functionalities have to be performed for thousands of different food‐derived molecules in order to identify important interactions. Our approach is to directly identify those food molecules that interact with cellular targets based on mass spectrometric (MS) techniques through immobilization of cellular target molecules on glass chips and incubation with various foods. Food‐derived molecules that bind with high affinity can then be directly analyzed by MS. We have chosen bovine colostrum, a potent bioactive food, and cellular receptors of the innate immune response as our model for proof of concept.