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Enhanced expression of glycine N ‐methyltransferase by adenovirus‐mediated gene transfer in CNS culture is neuroprotective
Author(s) -
Tsai MayJywan,
Chen YiMing Arthur,
Weng ChingFeng,
Liou DannYing,
Yang HsinChun,
Chen ChienHung,
Liao Roanna IHsin,
Kuo FuShan,
Chiu ChiuanWen,
Kuo HuaiSheng,
Huang MingChao,
Lin YiLo,
Lee MengJen,
Kuo WenChun,
Huang WenCheng,
Cheng Henrich
Publication year - 2010
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.05169.x
Subject(s) - biology , neuroprotection , microglia , microbiology and biotechnology , immunology , inflammation , neuroscience
Glycine N ‐methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus‐mediated transfer in cortical mixed neuron‐glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non‐neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad‐GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronal/oligodendroglial survival. Furthermore, these Ad‐GNMT‐infected cultures are significantly resistant to H 2 O 2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad‐GNMT‐infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal‐glial cultures is neuroprotective, most likely mediated through non‐neuronal cells.