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Oligonucleotide Probe Array for p53 Gene Alteration Analysis in DNA from Formalin‐Fixed Paraffin‐Embedded Breast Cancer Tissues
Author(s) -
Lumachi Franco,
Marino Filippo,
Varotto Sergio,
Basso Umberto
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04969.x
Subject(s) - oligonucleotide , microbiology and biotechnology , gene , exon , biology , polymerase chain reaction , dna sequencing , dna , oligomer restriction , mutation , breast cancer , cancer , genetics , cancer research
Mutations in the TP53 tumor‐suppressor ( p53 ) gene represent the most common molecular changes in various malignancies, including breast cancer (BC). We sequenced the p53 gene in DNA extracted from archival paraffin‐embedded BC tissues and compared the results obtained from direct sequencing with those obtained by oligonucleotide probe array (OPA). DNA was extracted from 34 samples. OPA correctly detected 13 genetic alterations in 14 cases, with a mutation frequency of 41.2%. No changes were detected in exons 3, 4, 9, 10, and 11 and no polymorphisms were found. Direct manual sequencing in which DNA was amplified by PCR showed 21 genetic mutations in 19 (55.9%) cases. Eight mutations were identified by both OPA and PCR methods. Although OPA detected fewer gene alterations than direct sequencing, the difference was not significant ( P = 0.11). In conclusion, the OPA may be safely used to identify individual genetic variations of human p53 gene in BC specimens.