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Role of Neutrophils in Arginine‐asymmetric Dimethylarginine Pathway Subsequent to Endotoxemia
Author(s) -
Nurten Türközkan,
Nihal Şahin,
Gonca Ozan,
Özgür Akgül,
Halil Yaman,
Filiz Bircan,
Barbaros Balabanli
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04951.x
Subject(s) - myeloperoxidase , arginine , asymmetric dimethylarginine , nitric oxide , chemistry , arginase , nitrotyrosine , peroxynitrite , pharmacology , medicine , nitric oxide synthase , enzyme , biochemistry , endocrinology , inflammation , superoxide , amino acid
There is a striking correlation between nitric oxide (NO) production and myeloperoxidase (MPO) enzyme activity accession in various tissues after endotoxemia. Both arginine–NO and arginine‐asymmetric dimethylarginine (ADMA) pathways have been recognized to play critical roles during infection and inflammation. We investigated whether there is a link between MPO‐mediated nitrating pathway and arginine–ADMA pathway after endotoxemia in liver. All experiments were performed in two groups (control and endotoxemia) of 10 guinea pigs. In this study, 6 h after the administration of endotoxin at a dose of 4 mg/kg, MPO activity and ADMA, L‐arginine, and 3‐nitrotyrosine (3‐NT, a stable product of peroxynitrite formation) levels were measured. Measurement of ADMA and L‐arginine were accomplished by HPLC with fluorescent detector. 3‐NT was quantified by HPLC with electrochemical detector. MPO activity was determined by spectrophotometric method. After administration of endotoxin, ADMA and L‐arginine levels decreased, but 3‐NT levels and MPO activity increased significantly. In conclusion, there is an inverse relationship between MPO‐mediated tyrosine nitration and arginine–ADMA pathway in liver after endotoxemia.