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Antigenic Phenotype and Phagocytic Capacity of MG‐63 Osteosarcoma Line
Author(s) -
DíazRodríguez L.,
GarcíaMartínez O.,
ArroyoMorales M.,
ReyesBotella C.,
Ruiz C.
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04950.x
Subject(s) - antigen , monoclonal antibody , osteosarcoma , cell culture , cd44 , osteoblast , pan t antigens , phenotype , antibody , microbiology and biotechnology , cell , flow cytometry , in vitro , chemistry , immunology , biology , cancer research , biochemistry , gene , genetics
Human osteoblasts isolated from bone tissue samples have their own specific antigen profile but also share expression of antigens that are characteristic of other immunocompetent cells. Given that these findings come from studies performed in primary cultures of human osteoblasts, it was decided to test whether these antigen profiles and functional characteristics are retained in a characterized osteoblast cell line (MG‐63). We show that some of these characteristics are also found in the MG‐63 osteosarcoma cell line. We have demonstrated, using monoclonal antibodies and cytometry, that these cells expressed CD10, CD13, CD44, and CD54 antigens but were negative for CD69 and HLA‐DR antigens. Functionally, 100% of MG‐63 cells showed phagocytic capacity with a high phagocytic index. This study corroborates that osteoblastic cells have an immunological profile.