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Distribution of Centromeric Proteins and PARP‐1 during Mitosis and Apoptosis
Author(s) -
Perdoni Federica,
Bottone Maria Grazia,
Soldani Cristiana,
Veneroni Paola,
Alpini Claudia,
Pellicciari Carlo,
Scovassi Anna Ivana
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04729.x
Subject(s) - mitosis , centromere , interphase , kinetochore , microbiology and biotechnology , poly adp ribose polymerase , cell cycle , biology , regulator , chemistry , dna , polymerase , apoptosis , genetics , gene , chromosome
A large complex of proteins, called CENPs, are associated with centromeric DNA. Some of them exhibit a cell cycle‐related expression (e.g., CENP‐E and ‐F) and are required for the transition from interphase to mitosis, whereas constitutive proteins (e.g., CENP‐A, ‐B, ‐C, ‐G, and ‐H) reside permanently at the centromere and are essential for the correct kinetochore assembly. Poly(ADP‐ribose) polymerase‐1 (PARP‐1), which plays an active role in many basic processes, was described as a possible regulator of CENPs. By multicolor immunofluorescence we therefore analyzed the distribution of PARP‐1 and its interaction with CENP‐B, ‐E, and ‐F during mitosis and apoptosis.