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Plant Polyphenols Effectively Protect HaCaT Cells from Ultraviolet C–Triggered Necrosis and Suppress Inflammatory Chemokine Expression
Author(s) -
Pastore Saveria,
Potapovich Alla,
Kostyuk Vladimir,
Mariani Valentina,
Lulli Daniela,
De Luca Chiara,
Korkina Liudmila
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04684.x
Subject(s) - verbascoside , proinflammatory cytokine , oxidative stress , chemistry , hacat , chemokine , pharmacology , immunology , biochemistry , inflammation , biology , glycoside , organic chemistry , in vitro , receptor
Oxidative stress is a common response of epidermal cells to a variety of noxious stimuli such as ultraviolet (UV) radiation from solar light and proinflammatory cytokines from skin‐infiltrating leukocytes. Here, we report that two types of plant‐derived antioxidants, the phenylpropanoid glycoside verbascoside as well as the flavonoids rutin and quercetin possess protective effects against UVC‐induced cell damage and proinflammatory activation. The molecules under investigation were effective against the loss of cell integrity associated with necrosis in doses consistent with their antioxidant activity, whereas they did not significantly oppose UVC‐induced proliferation arrest and apoptosis. By contrast, only verbascoside effectively inhibited cytokine‐induced release of proinflammatory mediators in a dose‐dependent fashion. Verbascoside and its homologue teupolioside dramatically impaired NF‐κB and AP‐1 DNA binding activity. These results suggest that plant polyphenols with antioxidant properties have distinct mechanisms in the suppression of oxidative stress induced in keratinocytes by different stimuli. Verbascoside and teupolioside are hence of potential interest in the protection of the skin from both environmental and inflammatory insults.