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In Vitro Effects of Antiphospholipid Syndrome‐IgG Fractions and Human Monoclonal Antiphospholipid IgG Antibody on Human Umbilical Vein Endothelial Cells and Monocytes
Author(s) -
Clemens Natascha,
Frauenknecht Katrin,
Katzav Aviva,
Sommer Clemens,
Von Landenberg Philipp
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04632.x
Subject(s) - umbilical vein , downregulation and upregulation , antiphospholipid syndrome , monocyte , monoclonal antibody , human umbilical vein endothelial cell , immunology , antibody , endothelial stem cell , stimulation , medicine , microbiology and biotechnology , biology , endocrinology , in vitro , biochemistry , gene
It has been shown that stimulation of endothelial cells and monocytes by antiphospholipid antibodies leads to a prothrombotic state involving upregulation of tissue factor (TF). We examined the in vitro effects of IgG fractions from patients with antiphospholipid syndrome (APS) and of a β‐2‐glycoprotein 1‐independent human monoclonal antiphospholipid antibody (HL‐5B) on human umbilical vein endothelial cells (HUVEC) in comparison to untreated cell controls and to exposure to monoclonal IgG control antibody. We also examined the effect of recombinant monocyte chemoattractant protein‐1 (MCP‐1) on peripheral blood monocytes. Stimulation of endothelial cells with APS IgG fractions or HL‐5B resulted in time‐dependent upregulation of MCP‐1 mRNA and protein expression. Stimulation with HL‐5B also led to time‐dependent upregulation of interleukin (IL)‐8 and intracellular adhesion molecule‐1 (ICAM‐1) mRNA and IL‐8 protein expressions. Stimulation of monocytes with recombinant MCP‐1 resulted in an upregulation of TF mRNA and TF protein. In conclusion these results might represent a mechanism for antiphospholipid antibody‐mediated thrombosis in APS patients.