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Immunohistochemical Localization of Hydroxysteroid Sulfotransferase and Sulfatase in the Brain of Rana esculenta Tadpoles
Author(s) -
Cevasco Alessandra,
Pittaluga Aurora,
Rego JeanLuc Do,
LuuThe Van,
Pelletier Georges,
Vaudry Hubert,
Mandich Alberta
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2009.04436.x
Subject(s) - neuroactive steroid , steroid sulfatase , sulfation , sulfotransferase , sulfatase , pregnenolone , pregnenolone sulfate , medicine , biology , olfactory bulb , endocrinology , immunohistochemistry , dehydroepiandrosterone , diencephalon , enzyme , biochemistry , central nervous system , receptor , hormone , steroid , androgen , gabaa receptor
Sulfation plays a major role in regulating the activity of various neurosteroids, including pregnenolone and dehydroepiandrosterone. The present report describes the immunohistochemical distribution of two enzymes involved in the control of neurosteroid sulfation, hydroxysteroid sulfotransferase (HST) and unconjugated steroid enzyme sulfatase (STS), in the brain of the European green frog Rana esculenta during development. HST and STS immunoreactivity were detected from stage VIII‐XII. At this early stage, HST‐positive fibers were seen in the glomerular layer and the basal rhombencephalon. Subsequently, at stage XIII‐XV, HST‐ and STS‐immunoreactive fibers were vizualized in the accessory olfactory bulb. At stage XVI‐XVIII, STS‐positive cell bodies were observed in the periventricular region of the diencephalon. These observations indicate that the enzymes controlling sulfation of hydroxysteroids are expressed in the frog brain during development.