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Laser Microdissection of Neonatal Porcine Endometrium for Tissue‐Specific Evaluation of Relaxin Receptor (RXFP1) Expression in Response to Perinatal Zearalenone Exposure
Author(s) -
Wiley Anne A.,
Kauffold Johannes,
Wähner Martin,
CreanHarris Bethany,
Miller Dori J.,
Bagnell Carol A.,
Bartol Frank F.
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2008.03821.x
Subject(s) - relaxin , laser capture microdissection , endometrium , estrogen receptor , biology , andrology , gene expression , receptor , reverse transcription polymerase chain reaction , estrogen , endocrinology , medicine , chemistry , gene , biochemistry , cancer , breast cancer
Porcine neonatal uterine relaxin receptor (RXFP1) expression is tissue compartment specific and estrogen sensitive. Here, procedures were established for laser microdissection, tissue capture, and quantification of the effects of perinatal exposure (14 days pre‐ to 21 days postnatal) to a selective estrogen receptor modulator of environmental origin, zearalenone (ZEA), on endometrial RXFP1 expression. Total RNA from captured endometrium was used to generate cDNA for quantitative reverse transcription‐PCR. Cycle threshold values indicated that ZEA reduced ( P < 0.06) endometrial RXFP1 expression on postnatal days 20–21.