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An in Vitro Study of the Protective Effect of Relaxin on Brain Tissue under Ischemic Stress
Author(s) -
Wilson Brian C.,
Rappaport Rebecca
Publication year - 2009
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2008.03806.x
Subject(s) - relaxin , in vitro , chemistry , brain tissue , microbiology and biotechnology , biophysics , pharmacology , medicine , biology , biochemistry , hormone
Organotypic brain slice culture was used to study the direct actions of relaxin on neural cells under ischemic stress. Cortical brain slices from neonatal rats were cultured for 14 days. Experimental slices were placed in a deoxygenated, glucose‐free balanced salt solution (BSS) for 1 h, one group with 10 −7 M H2 relaxin in the medium and the other without. Control slices were transferred to oxygenated BSS with glucose. Slices were returned to normal culture conditions and analyzed 1, 4, 8, and 24 h post‐treatment using propidium iodide (PI) fluorescence to highlight cellular damage or death. The percentages of dead and dying cells in a slice were compared between groups. Relaxin treatment attenuated PI staining at 4 and 8 h postischemia, suggesting a direct action(s) on cells that improves their chance of survival during ischemia.