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Acrylamide Stimulates Glutamine Uptake in Fischer 344 Rat Astrocytes by a Mechanism Involving Upregulation of the Amino Acid Transport System N
Author(s) -
WU QI,
SIDORYK MARTA,
MUTKUS LYSETTE,
ZIELIŃSKA MAGDALENA,
ALBRECHT JAN,
ASCHNER MICHAEL
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2005.tb00052.x
Subject(s) - acrylamide , glutamine , downregulation and upregulation , astrocyte , glioma , chemistry , amino acid , astrocytoma , biochemistry , microbiology and biotechnology , cancer research , biology , endocrinology , central nervous system , gene , organic chemistry , copolymer , polymer
A bstract : High demand of neoplastic tissues for glutamine (Gln) is met by its active transport across cell membranes. Chronic treatment with acrylamide in rodents is associated with an increased incidence of neoplasms, including astrocytomas. In this study, 24‐h acrylamide treatment significantly increased the initial rate of l‐[G‐ 3 H]glutamine uptake in astrocyte cultures derived from the acrylamide‐sensitive Fischer 344 rat, and this effect could be fully inhibited by histidine, a model substrate for the amino acid transport system N. RT‐PCR analysis revealed that acrylamide treatment caused a significant increase in the astrocytic expression of the mRNA coding for the major system N protein, SNAT3, which is specifically overexpressed in malignant gliomas in situ . The acrylamide‐induced upregulation of astrocytic Gln transport via system N is likely to affect Gln homeostasis in these cells and may be causally related to the increased astrocytoma incidence observed in Fischer 344 rats.