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In Vitro Infection of Nonendothelial Cells by Ehrlichia ruminantium
Author(s) -
ZWEYGARTH E.,
JOSEMANS A. I.
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb07429.x
Subject(s) - biology , vero cell , aborted fetus , andrology , microbiology and biotechnology , connective tissue , cell culture , fetus , virology , virus , genetics , pregnancy , medicine
A bstract : The Welgevonden stock of Ehrlichia ruminantium was propagated in eight nonendothelial cell cultures derived from different animal species, both ruminants and nonruminants. The origins of the cells were: bovine fetal testis (BFT), cat ovary (COC), donkey fibroblasts (DFC), sheep fibroblasts (E 2 ), horse testis (HTC), lamb fetal testis (LFT), mouse connective tissue (L), and African green monkey kidney (Vero). Four cell culture types (BFT, E 2 , LFT and Vero) required supplementation of the medium with cycloheximide for suitable growth of E. ruminantium , whereas the other four (COC, DFC, HTC, and L) did not. Three other stocks of E. ruminantium , Senegal, Ball 3, and Gardel, were also propagated, either in LFT cultures only or in both E 2 and LFT cell cultures. The Welgevonden stock was successfully initiated using E 2 and LFT cell cultures.