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‘ Candidatus Rickettsia tarasevichiae ’ in Ixodes persulcatus Ticks Collected in Russia
Author(s) -
SHPYNOV STANISLAV,
FOURNIER PIERREEDOUARD,
RUDAKOV NIKOLAY,
RAOULT DIDIER
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb07358.x
Subject(s) - ixodes persulcatus , rickettsia , biology , candidatus , ixodes , phylogenetic tree , 16s ribosomal rna , haemaphysalis , ixodidae , virology , tick , gene , genetics , virus
A bstract : We collected 209 Ixodes persulcatus ticks in various regions of Russia, including the southern Urals and western and eastern Siberia. Using PCR amplification and sequencing of the citrate synthase‐encoding gene ( glt A), we detected a new rickettsial genotype, which we named ‘ Candidatus Rickettsia tarasevichiae .’ This bacterium was found in 9.27%, 10.0%, and 20.5% of the ticks collected in western Siberia, eastern Siberia, and the southern Urals, respectively. ‘ Candidatus Rickettsia tarasevichiae ’ exhibited a 98% and 96% nucleotide sequence homology, with the 16S rDNA and glt A sequence, respectively, of R. canadensis , a rickettsia previously only found in Haemaphysalis leporispalustris ticks in North America. The phylogenetic analysis of ‘ Candidatus Rickettsia tarasevichiae ’ and other Rickettsia species allowed the creation of a new cluster with high bootstrap values within the Rickettsia genus involving this rickettsia, R. canadensis , and three uncultured rickettsiae from plant insects.