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Enzyme Transmission during Crossflow Filtration of Yeast Suspensions Using Gas/Liquid Two‐Phase Flows
Author(s) -
MERCIERBONIN MURIEL,
FONADE CHRISTIAN
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb06021.x
Subject(s) - microfiltration , chromatography , chemistry , filtration (mathematics) , fouling , slug flow , flux (metallurgy) , bubble , cross flow filtration , volumetric flow rate , permeation , membrane , membrane fouling , flow (mathematics) , two phase flow , thermodynamics , mechanics , biochemistry , statistics , mathematics , physics , organic chemistry
A bstract : The optimal conditions for recovery of an enzyme were determined using gas/liquid two‐phase flows. When filtering the enzyme‐only solution under single‐phase flow conditions, severe fouling occurred. This fouling was manifest as a decline in flux to less than 2% of the initial water flux and a decline in protein concentration in the permeate to 30% of its initial value, during a five‐hour filtration period. When yeast cells were added under the same experimental conditions, enzyme transmission was maintained at 100% for the five‐hour period and the enzyme mass flux was twofold higher. During gas‐sparged microfiltration of the enzyme/yeast mixture in a permeate‐recycling mode at the same liquid flow rate, gas/liquid slug flow strongly decreased the transmission of the enzyme (70% decrease), even though the permeate flux was improved (140% improvement). As a result, the mass flux of the enzyme was significantly reduced. However, with a bubble flow pattern, the permeate flux was 1.5 times higher and the transmission was maintained at a high level. The enzyme mass flux was then 25% higher when compared to single‐phase flow filtration conditions. During diafiltration experiments with a bubble flow pattern, a 13% higher enzyme recovery was achieved.