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Antibody W7C5 Defines a CD109 Epitope Expressed on CD34 + and CD34 − Hematopoietic and Mesenchymal Stem Cell Subsets
Author(s) -
GIESERT CHRISTINA,
MARXER ANKE,
SUTHERLAND D. ROBERT,
SCHUH ANDRE C.,
KANZ LOTHAR,
BÜHRING HANSJÖRG
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb03250.x
Subject(s) - cd34 , microbiology and biotechnology , antigen , epitope , stem cell , monoclonal antibody , antibody , haematopoiesis , biology , complementary dna , chemistry , immunology , biochemistry , gene
A bstract : The cell surface antigen recognized by monoclonal antibody W7C5 is expressed at low levels on human CD34 + and CD34 − bone marrow stem cell populations but at high levels on fetal liver CD34 + cells. To identify the recognized antigen, we performed partial amino acid sequence and MALDI analysis of purified W7C5 antigens. The analyses revealed that MoAb W7C5 detects CD109, a recently cloned glycophosphatidylinositol‐linked glycoprotein. The specificity of MoAb W7C5 was further confirmed by the selective recognition of CHO cells transfected with human CD109 cDNA.