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Early Stages of Hematopoietic Differentiation
Author(s) -
Spangrude Gerald J.,
Perry S. Scott,
Slayton William B.
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb03246.x
Subject(s) - progenitor cell , haematopoiesis , stem cell , biology , megakaryocyte , microbiology and biotechnology , transplantation , cellular differentiation , bone marrow , immunology , lineage markers , medicine , genetics , gene
A bstract : Mouse bone marrow contains hematopoietic stem cells as well as progenitor cells, which are partially differentiated offspring of stem cells. We have utilized several approaches to separate progenitors from stem cells in order to characterize essential differences between these two stages of development. As a first approach, we utilized the supravital fluorescent dye rhodamine‐123 (Rh‐123) to distinguish quiescent stem cells (Rh‐123 low ) from metabolically active progenitor cells (Rh‐123 hi ). Analysis of megakaryocyte potential in a tissue culture assay demonstrated that Rh‐123 hi progenitor cells were capable of robust megakaryocyte differentiation, while Rh‐123 low stem cells produced fewer colonies containing megakaryocytes. Transplantation of the two cell populations into irradiated recipients revealed the opposite outcome, suggesting that the tissue culture assay failed to predict behavior in a transplant setting. We also evaluated functional potential of lymphoid progenitors isolated by selecting for differential expression of Thy‐1.1 and c‐kit. The potential of defined cell populations to differentiate as T or B lymphocytes in vivo was dependent upon the time post transplant at which animals were evaluated. These studies underscore the need for caution in the interpretation of lineage potentials evaluated by both in vitro and in vivo assays.