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Expression, Desensitization, and Internalization of the ACTH Receptor (MC2R)
Author(s) -
CLARK ADRIAN J.L.,
BAIG ASMA H.,
NOON LUKE,
SWORDS FRANCESCA M.,
HUNYADY LASZLO,
KING PETER J.
Publication year - 2003
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2003.tb03169.x
Subject(s) - internalization , desensitization (medicine) , receptor , homologous desensitization , acth receptor , microbiology and biotechnology , chemistry , adrenocorticotropic hormone , endocrinology , medicine , biology , hormone
A bstract : Research into the functions and mechanisms of action of the melanocortin 2 receptor (MC2R) has been severely hampered by difficulties in expressing this gene in heterologous cells. This probably arises because of the need for a cofactor for cell surface expression. Using either the Y1 cell line that expresses endogenous MC2R or the Y6 cell line that expresses this putative expression factor, we have explored the mechanisms of desensitization and internalization after agonist stimulation. Protein kinase A dependence of desensitization has been demonstrated, although internalization is apparently independent of this kinase and dependent on a G protein receptor kinase. Possible underlying reasons for this paradox are discussed.