Premium
Analysis of in Vivo Endothelial Cell Activation Applying RT‐PCR following Endothelial Cell Isolation by Laser Dissection Microscopy
Author(s) -
ÁSGEIRSDÓTTIR S. A.,
WERNER N.,
HARMS G.,
BERG A.,
MOLEMA G.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04704.x
Subject(s) - cd31 , in vivo , endothelial stem cell , microbiology and biotechnology , pathology , dissection (medical) , h&e stain , cell , microscopy , gene expression , chemistry , staining , biology , anatomy , gene , immunohistochemistry , medicine , in vitro , biochemistry
A bstract : Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF‐α. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT‐PCR for GAPDH, CD31, VCAM‐1, ICAM‐1, and E‐selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.