Premium
Effects of Homocysteine on the Cellular Responses of Murine Endothelial Cells Cultured in Vitro
Author(s) -
TACHENY A.,
SANTOS S. DOS,
HUET A.C.,
STEENBRUGGE M. VAN,
MICHIELS C.,
HOLVOET P.,
RAES M.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04699.x
Subject(s) - homocysteine , proinflammatory cytokine , in vitro , methionine , tumor necrosis factor alpha , microbiology and biotechnology , messenger rna , chemistry , biology , gene , inflammation , biochemistry , immunology , amino acid
A bstract : Homocysteine, derived from the metabolism of methionine, is claimed as a proatherogenic factor that leads to vascular dysfunction. To gain better insight into the molecular mechanisms involved, homocysteine was tested on a model of murine endothelial cells cultured in vitro , using a prototype DNA chip. The DNA chip was designed to follow the expression at the mRNA level of some major proinflammatory genes; TNF‐α was used as a positive control.