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Detection and Characterization of Membrane‐Bound Selenoproteins in the Kidney of the Rat
Author(s) -
KYRIAKOPOULOS A.,
BERTELSMANN H.,
GRAEBERT A.,
HOPPE B.,
KÜHBACHER M.,
BEHNE D.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04652.x
Subject(s) - selenium , membrane , chemistry , biochemistry , cell fractionation , microsome , membrane protein , molecular mass , in vivo , biology , in vitro , enzyme , microbiology and biotechnology , organic chemistry
A bstract : The essential trace element selenium is of fundamental importance to human biology. Information on the membrane‐bound selenoproteins was obtained by labeling of rats in vivo with 75 Se‐selenite, subcellular fractionation of the tissue homogenates, separation of the proteins by SDS‐PAGE, two‐dimensional electrophoresis, and selenium detection by autoradiography. In this way, in addition to already known membrane‐bound selenoproteins three novel selenoproteins in the molecular mass range of 38–40, 18, and 12–13 kDa were detected in the microsomal membranes.