Premium
Prodigiosin Induces Caspase‐9 and Caspase‐8 Activation and Cytochrome C Release in Jurkat T Cells
Author(s) -
MONTANER BEATRIZ,
PÉREZTOMÁS RICARDO
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04642.x
Subject(s) - caspase , poly adp ribose polymerase , cytochrome c , jurkat cells , apoptosis , microbiology and biotechnology , chemistry , cleavage (geology) , cytosol , prodigiosin , caspase 3 , intrinsic apoptosis , biology , biochemistry , programmed cell death , dna , polymerase , enzyme , immunology , t cell , paleontology , immune system , escherichia coli , fracture (geology) , gene , serratia marcescens
A bstract : Prodigiosin (PG) is an active component of bacterial origin, with reported apoptotic effects. We examined the activation of caspases‐9, ‐8, and ‐3 in PG‐treated Jurkat cells in a dose‐response study. These caspases were activated in apoptotic cells, as judged by the appearance of cleavage products from procaspases, and cytochrome c (cyt c) was released to the cytosol. In addition, PG induced the cleavage of poly (ADP‐ribose) polymerase (PARP). This study demonstrates that the activation of caspases‐9, ‐8, and ‐3 mediates PG‐induced apoptosis.