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Space Flight and Insulin‐like Growth Factor‐I Signaling in Rat Osteoblasts
Author(s) -
KUMEI YASUHIRO,
NAKAMURA HIROSHI,
MORITA SADAO,
AKIYAMA HIDEO,
HIRANO MASAHIKO,
OHYA KEI'ICHI,
SHINOMIYA KEN'ICHI,
SHIMOKAWA HITOYATA
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04609.x
Subject(s) - insulin like growth factor , growth factor , microbiology and biotechnology , space (punctuation) , osteoblast , insulin , chemistry , endocrinology , medicine , biology , computer science , biochemistry , receptor , in vitro , operating system
A bstract : The primary culture of rat osteoblasts was treated with 1α,25 dihydroxyvitamin D 3 and fixed with guanidine isothiocyanate solution during a space shuttle flight. The mRNA levels were analyzed by quantitative reverse transcription‐polymerase chain reaction. Microgravity decreased the mRNA levels of insulin‐like growth factor‐I (IGF‐I) and increased reciprocally the IGF‐I receptor mRNA levels, as compared to the ground (1 × g ) control. Microgravity completely suppressed the mRNA expression of the insulin receptor substrate‐1, the postreceptor signaling molecule of IGF‐I. Microgravity might deteriorate the action and signaling of IGF‐I in rat osteoblasts.