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Vasostatins Exert Negative Inotropism in the Working Heart of the Frog
Author(s) -
CORTI A.,
MANNARINO C.,
MAZZA R.,
COLOMBO B.,
LONGHI R.,
TOTA B.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04497.x
Subject(s) - isoprenaline , medicine , muscarinic acetylcholine receptor , endocrinology , rana , basal (medicine) , adrenergic receptor , chemistry , in vitro , receptor , biology , stimulation , biochemistry , insulin
A bstract : An in vitro isolated working frog heart ( Rana esculenta ) was used to study the effects of exogenous CGA 1‐76 (vasostatin 1), CGA 1‐113 (vasostatin 2), and the synthetic CGA 7‐57 on cardiac performance. Under basal cardiac conditions, the dose‐response curves of the three peptides from 10 −8 to 10 −7 M showed a significant calcium‐dependent negative inotropism that involved neither the endocardial endothelium nor the adrenergic and muscarinic receptors. In addition, the CgA fragments clearly counteracted the typical positive inotropism of isoprenaline (10 −<9 M). Taken together, these results provide the first evidence for a cardio‐suppressive role for the vasostatins.