Premium
Separating Vesicle Fusion and Exocytosis in Hypertonic Conditions
Author(s) -
TROYER KEVIN P.,
MUNDORF MICHELLE L.,
FRIES HARVEY E.,
WIGHTMAN R. MARK
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04470.x
Subject(s) - exocytosis , osmotic concentration , vesicle , chemistry , tonicity , osmotic shock , biophysics , microbiology and biotechnology , extracellular , biochemistry , biology , membrane , gene
A bstract : The existence of an osmotic gradient between the vesicular contents of chromaffin and mast cells and the extracellular fluid plays a key role in the exocytotic process. Altering this gradient by elevating the external buffer osmolarity allows for the inhibition of exocytosis from cells and isolation and identification of prespike current events upon stimulation using cyclic voltammetry. Subsequent replacement of the osmotic gradient leads to release of the contents from fused vesicles.