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In Situ Chelation of Ca 2+ in Intracellular Stores Induces Capacitative Ca 2+ Entry in Bovine Adrenal Chromaffin Cells
Author(s) -
POWIS DAVID A.,
ZERBES MARIANN
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04452.x
Subject(s) - intracellular , cytosol , serca , chelation , chemistry , second messenger system , medicine , endocrinology , biophysics , biology , biochemistry , enzyme , atpase , organic chemistry
A bstract : Reduction of intracellular Ca 2+ stores (by chelation with the EDTA structural analogue, TPEN) is itself a sufficient trigger of capacitative Ca 2+ entry. TPEN may be a useful tool in studies to investigate the mechanism of capacitative Ca 2+ entry, since potential confounders, such as changes in cytosolic Ca 2+ and second messenger levels, are obviated and the use of pharmacological modifiers of SERCA pumps and Ca 2+ release channels is avoided.