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Hypoxia Activates Multiple Transcriptional Pathways in Mouse Pheochromocytoma Cells
Author(s) -
EVINGER MARIAN J.,
CIKOS STEFAN,
NWAFORANENE VICTORIA,
POWERS JAMES F.,
TISCHLER ARTHUR S.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04434.x
Subject(s) - hypoxia (environmental) , gene , transcription factor , messenger rna , pheochromocytoma , microbiology and biotechnology , transcription (linguistics) , intermittent hypoxia , biology , catecholamine , endocrinology , medicine , chemistry , genetics , linguistics , philosophy , organic chemistry , oxygen , obstructive sleep apnea
A bstract : Mouse pheochromocytoma cells (MPCs) provide an excellent model system for investigating the effects of hypoxia on catecholamine enzyme genes and on transcription factors mediating stress responses. RT‐PCR detects rapid, transient increases in PNMT mRNA in hypoxic MPC 712 cells. Additionally, elevation of mRNAs encoding transcription factors hypoxia inducible factor 1 (HIF‐1) α subunit and Egr‐1 are evident within 60 min incubation in anoxia. Therefore, hypoxia elicits rapid transcriptional responses in numerous genes expressed by chromaffin cells.