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Genetic Immunization with Ehrlichia ruminantium GroEL and GroES Homologues
Author(s) -
PRETORIUS ALRI,
STRIJP F.,
BRAYTON K. A.,
COLLINS N. E.,
ALLSOPP B. A.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04368.x
Subject(s) - groes , groel , biology , splenocyte , virology , gene , immunization , salmonella , dna vaccination , microbiology and biotechnology , antigen , genetics , bacteria , escherichia coli
A bstract :Ehrlichia ruminantium GroEL and GroES genes were amplified from E. ruminantium Welgevonden genomic DNA and were cloned into genetic vaccine and Salmonella expression vectors. These constructs were used to inoculate Balb/c and C57BL/6J mice. Both GroEL and GroES induced low levels of protection in Balb/c and C57BL/6J mice immunized with the Salmonella expression vectors. None of the mice inoculated with the genetic vaccine survived. Immunological memory was also tested in these mice and a correlation between splenocyte proliferation and the survival rate was observed.