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Expression of HIP/PAP mRNA in Human Hepatoma Cell Lines
Author(s) -
CERVELLO M.,
GIANNITRAPANI L.,
ROSA M.,
NOTARBARTOLO M.,
D'ALESSANDRO N.,
VIRRUSO L.,
IOVANNA J. L.,
MONTALTO G.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04094.x
Subject(s) - pancreas , messenger rna , hepatocellular carcinoma , gene , polymerase chain reaction , reverse transcriptase , biology , gene expression , reverse transcription polymerase chain reaction , cell culture , cancer research , microbiology and biotechnology , genetics , endocrinology
A bstract : The present study attempts to shed more light on the role of hepatocarcinoma‐intestine‐pancreas/pancreatic associated protein (HIP/PAP) in hepatoma cells. We initially examined, by reverse transcription‐polymerase chain reaction (RT‐PCR), the HIP/PAP transcripts present in human hepatoma cell lines of different origins and with different grades of differentiation and genetic profiles. We also used DNA sequencing analysis to investigate the structure of the HIP/PAP gene. Further investigation is necessary to define the role of HIP/PAP during the development of human hepatocellular carcinoma and to ascertain whether the use of different transcripts is helpful in regulating HIP/PAP expression in transformed liver cells.