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Contradictory Effects of Sodium Nitroprusside and S ‐Nitroso‐ N ‐Acetylpenicillamine on Oxidative Stress in Brain Dopamine Neurons in Vivo
Author(s) -
RAUHALA PEKKA,
ANDOH TSUGUNOBU,
YEH KEVIN,
CHIUEH CHUANG C.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb04056.x
Subject(s) - chemistry , sodium nitroprusside , substantia nigra , oxidative stress , nitric oxide , neuroprotection , dopamine , lipid peroxidation , ferrous , in vivo , pars compacta , snap , pharmacology , biochemistry , dopaminergic , endocrinology , medicine , biology , computer graphics (images) , microbiology and biotechnology , organic chemistry , computer science
A bstract : To investigate whether nitric oxide (•NO) is neurotoxic or neuroprotective in the brain, we compared the in vivo role of S ‐nitroso‐ N ‐acetylpenicillamine (SNAP) with that of sodium nitroprusside (SNP) on ferrous citrate‐induced oxidative stress and neuronal loss in the rat nigrostriatal dopaminergic system. It is known that light irradiation releases •NO from its donor compounds; these irradiated •NO donors were used as sham controls in this study. Intranigral infusion of ferrous citrate (4.2 nmol) into the rat midbrain substantia nigra compacta area caused acute lipid peroxidation in the substantia nigra and chronic dopamine depletion in the caudate nucleus. Coinfusion of freshly prepared SNAP (0‐8.4 nmol) or •NO (about 2 nmol), but not SNP, rescued iron‐induced dopamine depletion in the rat brain in vivo . In fact, SNP produced prooxidative effects similar to ferrous citrate both in vivo and in vitro , since SNP is a redox iron complex. Consistently, •NO and SNAP inhibited, whereas SNP potentiated, •OH generation and lipid peroxidation evoked by ferrous citrate in vitro . We previously reported that freshly prepared, but not irradiated, S ‐nitroso‐l‐glutathione (GSNO) protected brain dopamine neurons against oxidative stress in vivo . As well as these antioxidative properties, our recent reports (see Ref. 1) indicate that •NO/GSNO activated guanylyl cyclase, increased cGMP and that could lead to PKG‐mediated expression of MnSOD, Bcl‐2, and thioredoxin for preconditioning neuroprotection against 1‐methyl‐4‐phenylpyridinium (MPP + ).1 In conclusion, •NO and S ‐nitrosothiols (e.g., GSNO and SNAP) can scavenge reactive oxygen species and activate the heme moiety of guanylyl cyclase, resulting in protection of brain dopamine neurons through both antioxidative and antiapoptotic mechanisms.