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Different Osteochondral Potential of Clonal Cell Lines Derived from Adult Human Trabecular Bone
Author(s) -
OSYCZKA ANNA M.,
NÖTH ULRICH,
DANIELSON KEITH G.,
TUAN ROCKY S.
Publication year - 2002
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1111/j.1749-6632.2002.tb03054.x
Subject(s) - mesenchymal stem cell , adipogenesis , progenitor cell , chondrogenesis , microbiology and biotechnology , biology , human bone , population , transduction (biophysics) , cell , cell culture , stem cell , in vitro , genetics , medicine , botany , environmental health
A bstract : Cells derived from human trabecular bones have been shown to have multipotential differentiation ability along osteogenic, chondrogenic, and adipogenic lineages. In this study, we have derived two clonal sublines of human trabecular bone cells by means of stable transduction with human papilloma virus E6/E7 genes. Our results showed that these clonal sublines differ in their osteochondral potential, but are equally adipogenic, indicative of the heterogeneous nature of the parental cell population. The availability of these cell lines should be useful for the analysis of the mechanisms regulating the differentiation of adult mesenchymal progenitor cells.